2x PCR PreMix, with dye

MB000067-EQ2: 2x PCR PreMix, with dye

The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.

The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

Features of the Taq DNA polymerases contained in the PCR master mix:

● High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
● High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
● Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
● Terminal transferase activity of adding a single nucleotide (adenosine) at 3’ end of the extension product, facilitating TA cloning of PCR products.

● High efficiency with 30'  per kb DNA  extension time.

● Easily amplify DNA fragments up to 5 kb.
● High purity. >98%,  No contamination detected in standard PCR test reactions.
● Reproducible results.

The Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. The Taq DNA Polymerase has 5´→3´ polymerase activity, double-strand specific 5´→3´ exonuclease activity, and 3' adenylation activity.

Unit definition: One unit of the Taq DNA polymerase means incorporating 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 °C.

Applications of the Taq DNA polymerase:
● Routine PCR using genomic, viral and plasmid templates.
● RT-PCR.
● DNA cloning and subcloning.
● Primer extension.
● DNA and colony screening.
● Genotyping.
● DNA mutagenesis.
● Generating hybridization probes for Southern or Northern hybridization.

Concentration: 5 units/µl